SNAP annotations: PDB entry 8toc

PDB-id

8toc; DSSR-derived features in text and JSON formats; DNAproDB

Class

virus-RNA

Method

cryo-EM (3.11 Å)

Summary

Acinetobacter phage ap205

Reference

Meng R, Xing Z, Chang JY, Yu Z, Thongchol J, Xiao W, Wang Y, Chamakura K, Zeng Z, Wang F, Young R, Zeng L, Zhang J (2024): "Structural basis of Acinetobacter type IV pili targeting by an RNA virus." Nat Commun, 15, 2746. doi: 10.1038/s41467-024-47119-5.

Abstract

Acinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. Single-stranded RNA bacteriophages target the bacterial retractile pili, including type IV. Our study delves into the interaction between Acinetobacter phage AP205 and type IV pili. Using cryo-electron microscopy, we solve structures of the AP205 virion with an asymmetric dimer of maturation proteins, the native Acinetobacter type IV pili bearing a distinct post-translational pilin cleavage, and the pili-bound AP205 showing its maturation proteins adapted to pilin modifications, allowing each phage to bind to one or two pili. Leveraging these results, we develop a 20-kilodalton AP205-derived protein scaffold targeting type IV pili in situ, with potential for research and diagnostics.