Summary information and primary citation
- PDB-id
-
8eup;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- ribosome
- Method
- cryo-EM (3.1 Å)
- Summary
- Ytm1 associated 60s nascent ribosome state 1a
- Reference
-
Ugolini I, Bilokapic S, Ferrolino M, Teague J, Yan Y,
Zhou X, Deshmukh A, White M, Kriwacki RW, Halic M (2022):
"Chromatin
localization of nucleophosmin organizes ribosome
biogenesis." Mol.Cell, 82,
4443. doi: 10.1016/j.molcel.2022.10.033.
- Abstract
- Ribosome biogenesis takes place in the nucleolus, a
nuclear membrane-less organelle. Although well studied, it
remains unknown how nascent ribosomal subunits separate
from the central chromatin compartment and move to the
outer granular component, where maturation occurs. We find
that the Schizosaccharomyces pombe nucleophosmin-like
protein Fkbp39 localizes to rDNA sites encoding the 60S
subunit rRNA, and this localization contributes to its
specific association with nascent 60S subunits. Fkbp39
dissociates from chromatin to bind nascent 60S subunits,
causing the latter to partition away from chromatin and
from nascent 40S subunits through liquid-liquid phase
separation. In vivo, Fkbp39 binding directs the
translocation of nascent 60S subunits toward the
nucleophosmin-rich granular component. This process
increases the efficiency of 60S subunit assembly,
facilitating the incorporation of 60S RNA domain III. Thus,
chromatin localization determines the specificity of
nucleophosmin in sorting nascent ribosomal subunits and
coordinates their movement into specialized assembly
compartments within the nucleolus.
