Summary information and primary citation
- PDB-id
-
7n0b;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- viral protein-RNA
- Method
- cryo-EM (3.9 Å)
- Summary
- cryo-EM structure of sars-cov-2 nsp10-nsp14 (wt)-RNA
complex
- Reference
-
Liu C, Shi W, Becker ST, Schatz DG, Liu B, Yang Y (2021):
"Structural
basis of mismatch recognition by a SARS-CoV-2
proofreading enzyme." Science,
373, 1142-1146. doi: 10.1126/science.abi9310.
- Abstract
- Coronavirus 3′-to-5′ exoribonuclease (ExoN), residing
in the nonstructural protein (nsp) 10–nsp14 complex, boosts
replication fidelity by proofreading RNA synthesis and is
critical for the virus life cycle. ExoN also recognizes and
excises nucleotide analog inhibitors incorporated into the
nascent RNA, undermining the effectiveness of nucleotide
analog–based antivirals. Here we present cryo–electron
microscopy structures of both wild-type and mutant severe
acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
nsp10-nsp14 in complex with an RNA substrate bearing a
3′-end mismatch at resolutions ranging from 2.5 to 3.9
angstroms. The structures reveal the molecular determinants
of ExoN substrate specificity and offer insight into the
molecular mechanisms of mismatch correction during
coronavirus RNA synthesis. Our findings provide guidance
for rational design of improved anticoronavirus
therapies.
