Summary information and primary citation
- PDB-id
-
5lms;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- ribosome
- Method
- cryo-EM (5.1 Å)
- Summary
- Structure of bacterial 30s-if1-if3-mrna-trna
translation pre-initiation complex(state-2c)
- Reference
-
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan
V (2016): "Large-Scale
Movements of IF3 and tRNA during Bacterial Translation
Initiation." Cell, 167,
133-144.e13. doi: 10.1016/j.cell.2016.08.074.
- Abstract
- In bacterial translational initiation, three initiation
factors (IFs 1-3) enable the selection of initiator tRNA
and the start codon in the P site of the 30S ribosomal
subunit. Here, we report 11 single-particle cryo-electron
microscopy (cryoEM) reconstructions of the complex of
bacterial 30S subunit with initiator tRNA, mRNA, and IFs
1-3, representing different steps along the initiation
pathway. IF1 provides key anchoring points for IF2 and IF3,
thereby enhancing their activities. IF2 positions a domain
in an extended conformation appropriate for capturing the
formylmethionyl moiety charged on tRNA. IF3 and tRNA
undergo large conformational changes to facilitate the
accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet))
into the P site for start codon recognition.
