SNAP annotations: PDB entry 4lt8

PDB-id

4lt8; DSSR-derived features in text and JSON formats; DNAproDB

Class

ribosome

Method

X-ray (3.14 Å)

Summary

Crystal structure of trna proline (cgg) bound to codon ccc-g on the ribosome

Reference

Maehigashi T, Dunkle JA, Miles SJ, Dunham CM (2014): "Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops." Proc.Natl.Acad.Sci.USA, 111, 12740-12745. doi: 10.1073/pnas.1409436111.

Abstract

Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNA(SufA6) and N1-methylguanosine at position 37 (m(1)G37) modification-deficient anticodon stem loop(Pro), both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNA(SufA6) to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m(1)G37 modification of tRNA(Pro) also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame.