Summary information and primary citation
- PDB-id
-
3g9m;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (1.61 Å)
- Summary
- Gr DNA-binding domain:sgk 16bp complex-44
- Reference
-
Meijsing SH, Pufall MA, So AY, Bates DL, Chen L, Yamamoto
KR (2009): "DNA
binding site sequence directs glucocorticoid receptor
structure and activity." Science,
324, 407-410. doi: 10.1126/science.1164265.
- Abstract
- Genes are not simply turned on or off, but instead
their expression is fine-tuned to meet the needs of a cell.
How genes are modulated so precisely is not well
understood. The glucocorticoid receptor (GR) regulates
target genes by associating with specific DNA binding
sites, the sequences of which differ between genes.
Traditionally, these binding sites have been viewed only as
docking sites. Using structural, biochemical, and
cell-based assays, we show that GR binding sequences,
differing by as little as a single base pair,
differentially affect GR conformation and regulatory
activity. We therefore propose that DNA is a
sequence-specific allosteric ligand of GR that tailors the
activity of the receptor toward specific target genes.
