Summary information and primary citation
- PDB-id
-
1zse;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- RNA-virus-viral protein
- Method
- X-ray (3.0 Å)
- Summary
- RNA stemloop from bacteriophage qbeta complexed with an
n87s mutant ms2 capsid
- Reference
-
Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H,
Adams CJ, Peabody DS, Phillips SEV, Stonehouse NJ, Liljas
L, Stockley PG (2006): "Structural
Basis of RNA Binding Discrimination between
Bacteriophages Qbeta and MS2." Structure,
14, 487-495. doi: 10.1016/j.str.2005.12.006.
- Abstract
- Sequence-specific interactions between RNA stem-loops
and coat protein (CP) subunits play vital roles in the life
cycles of the RNA bacteriophages, e.g., by allowing
translational repression of their replicase cistrons and
tagging their own RNA genomes for encapsidation. The CPs of
bacteriophages Qbeta and MS2 each discriminate in favor of
their cognate translational operators, even in the presence
of closely related operators from other phages in vivo.
Discrete mutations within the MS2 CP have been shown to
relax this discrimination in vitro. We have determined the
structures of eight complexes between such mutants and both
MS2 and Qbeta stem-loops with X-ray crystallography. In
conjunction with previously determined in vivo repression
data, the structures enable us to propose the molecular
basis for the discrimination mechanism.
