SNAP annotations: PDB entry 1yyo

PDB-id

1yyo; DSSR-derived features in text and JSON formats; DNAproDB

Class

hydrolase-RNA

Method

X-ray (2.9 Å)

Summary

Crystal structure of rnase iii mutant e110k from aquifex aeolicus complexed with double-stranded RNA at 2.9-angstrom resolution

Reference

Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X (2005): "Intermediate states of ribonuclease III in complex with double-stranded RNA." Structure, 13, 1435-1442. doi: 10.1016/j.str.2005.06.014.

Abstract

Bacterial ribonuclease III (RNase III) can affect RNA structure and gene expression in either of two ways: as a processing enzyme that cleaves double-stranded (ds) RNA, or as a binding protein that binds but does not cleave dsRNA. We previously proposed a model of the catalytic complex of RNase III with dsRNA based on three crystal structures, including the endonuclease domain of RNase III with and without bound metal ions and a dsRNA binding protein complexed with dsRNA. We also reported a noncatalytic assembly observed in the crystal structure of an RNase III mutant, which binds but does not cleave dsRNA, complexed with dsRNA. We hypothesize that the RNase III*dsRNA complex can exist in two functional forms, a catalytic complex and a noncatalytic assembly, and that in between the two forms there may be intermediate states. Here, we present four crystal structures of RNase III complexed with dsRNA, representing possible intermediates.