SNAP annotations: PDB entry 1rlg

PDB-id

1rlg; DSSR-derived features in text and JSON formats; DNAproDB

Class

structural protein-RNA

Method

X-ray (2.7 Å)

Summary

Molecular basis of box c-d RNA-protein interaction: co-crystal structure of the archaeal srnp intiation complex

Reference

Moore T, Zhang Y, Fenley MO, Li H (2004): "Molecular Basis of Box C/D RNA-Protein Interactions; Cocrystal Structure of Archaeal L7Ae and a Box C/D RNA." STRUCTURE, 12, 807-818. doi: 10.1016/j.str.2004.02.033.

Abstract

We have determined and refined a crystal structure of the initial assembly complex of archaeal box C/D sRNPs comprising the Archaeoglobus fulgidus (AF) L7Ae protein and a box C/D RNA. The box C/D RNA forms a classical kink-turn (K-turn) structure and the resulting protein-RNA complex serves as a distinct platform for recruitment of the fibrillarin-Nop5p complex. The cocrystal structure confirms previously proposed secondary structure of the box C/D RNA that includes a protruded U, a UU mismatch, and two sheared tandem GA base pairs. Detailed structural comparisons of the AF L7Ae-box C/D RNA complex with previously determined crystal structures of L7Ae homologs in complex with functionally distinct K-turn RNAs revealed a set of remarkably conserved principles in protein-RNA interactions. These analyses provide a structural basis for interpreting the functional roles of the box C/D sequences in directing specific assembly of box C/D sRNPs.