Summary information and primary citation
- PDB-id
-
1kuq;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- ribosome
- Method
- X-ray (2.84 Å)
- Summary
- Crystal structure of t3c mutant s15 ribosomal protein
in complex with 16s rrna
- Reference
-
Revtovich SV, Nikulin AD, Nikonov SV (2004): "Role of
N-terminal helix in interaction of ribosomal protein S15
with 16S rRNA." Biochemistry Mosc.,
69, 1319-1323. doi: 10.1007/s10541-005-0076-5.
- Abstract
- The position and conformation of the N-terminal helix
of free ribosomal protein S15 was earlier found to be
modified under various conditions. This variability was
supposed to provide the recognition by the protein of its
specific site on 16S rRNA. To test this hypothesis, we
substituted some amino acid residues in this helix and
assessed effects of these substitutions on the affinity of
the protein for 16S rRNA. The crystal structure of the
complex of one of these mutants (Thr3Cys S15) with the 16S
rRNA fragment was determined, and a computer model of the
complex containing another mutant (Gln8Met S15) was
designed. The available and new information was analyzed in
detail, and the N-terminal helix was concluded to play no
significant role in the specific binding of the S15 protein
to its target on 16S rRNA.
