Summary information and primary citation
- PDB-id
-
1k1g;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- gene regulation-RNA
- Method
- NMR
- Summary
- Structural basis for recognition of the intron branch
site RNA by splicing factor 1
- Reference
-
Liu Z, Luyten I, Bottomley MJ, Messias AC,
Houngninou-Molango S, Sprangers R, Zanier K, Kramer A,
Sattler M (2001): "Structural
basis for recognition of the intron branch site RNA by
splicing factor 1." Science,
294, 1098-1102. doi: 10.1126/science.1064719.
- Abstract
- During spliceosome assembly, splicing factor 1 (SF1)
specifically recognizes the intron branch point sequence
(BPS) UACUAAC in the pre-mRNA transcripts. We show that the
KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K)
fold which is necessary and sufficient for BPS binding. The
3' part of the BPS (UAAC), including the conserved branch
point adenosine (underlined), is specifically recognized in
a hydrophobic cleft formed by the Gly-Pro-Arg-Gly motif and
the variable loop of the KH domain. The QUA2 region
recognizes the 5' nucleotides of the BPS (ACU). The branch
point adenosine acting as the nucleophile in the first
biochemical step of splicing is deeply buried. BPS RNA
recognition suggests how SF1 may facilitate subsequent
formation of the prespliceosomal complex A.
