SNAP annotations: PDB entry 1j59

PDB-id

1j59; DSSR-derived features in text and JSON formats; DNAproDB

Class

gene regulation-DNA

Method

X-ray (2.5 Å)

Summary

Catabolite gene activator protein (cap)-DNA complex + adenosine-3',5'-cyclic-monophosphate

Reference

Parkinson G, Wilson C, Gunasekera A, Ebright YW, Ebright RE, Berman HM (1996): "Structure of the CAP-DNA complex at 2.5 angstroms resolution: a complete picture of the protein-DNA interface." J.Mol.Biol., 260, 395-408. doi: 10.1006/jmbi.1996.0409.

Abstract

The crystallographic structure of the CAP-DNA complex at 3.0 A resolution has been reported previously. For technical reasons, the reported structure had been determined using a gapped DNA molecule lacking two phosphates important for CAP-DNA interaction. In this work, we report the crystallographic structure of the CAP-DNA complex at 2.5 A resolution using a DNA molecule having all phosphates important for CAP-DNA interaction. The present resolution permits unambiguous identification of amino acid-base and amino acid-phosphate hydrogen bonded contacts in the CAP-DNA complex. In addition, the present resolution permits accurate definition of the kinked DNA conformation in the CAP-DNA complex.