Summary information and primary citation
- PDB-id
-
1ig9;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- transferase-DNA
- Method
- X-ray (2.6 Å)
- Summary
- Structure of the replicating complex of a pol alpha
family DNA polymerase
- Reference
-
Franklin MC, Wang J, Steitz TA (2001): "Structure
of the Replicating Complex of a Pol Alpha Family DNA
Polymerase." Cell(Cambridge,Mass.),
105, 657-667. doi: 10.1016/S0092-8674(01)00367-1.
- Abstract
- We describe the 2.6 A resolution crystal structure of
RB69 DNA polymerase with primer-template DNA and dTTP,
capturing the step just before primer extension. This
ternary complex structure in the human DNA polymerase alpha
family shows a 60 degrees rotation of the fingers domain
relative to the apo-protein structure, similar to the
fingers movement in pol I family polymerases. Minor groove
interactions near the primer 3' terminus suggest a common
fidelity mechanism for pol I and pol alpha family
polymerases. The duplex product DNA orientation differs by
40 degrees between the polymerizing mode and editing mode
structures. The role of the thumb in this DNA motion
provides a model for editing in the pol alpha family.
