Summary information and primary citation
- PDB-id
-
1if1;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (3.0 Å)
- Summary
- Interferon regulatory factor 1 (irf-1) complex with
DNA
- Reference
-
Escalante CR, Yie J, Thanos D, Aggarwal AK (1998):
"Structure
of IRF-1 with bound DNA reveals determinants of
interferon regulation." Nature,
391, 103-106. doi: 10.1038/34224.
- Abstract
- The family of interferon regulatory factor (IRF)
transcription factors is important in the regulation of
interferons in response to infection by virus and in the
regulation of interferon-inducible genes. The IRF family is
characterized by a unique 'tryptophan cluster' DNA-binding
region. Here we report the crystal structure of the IRF-1
region bound to the natural positive regulatory domain I
(PRD I) DNA element from the interferon-beta promoter. The
structure provides the first three-dimensional view of a
member of the growing IRF family, revealing a new
helix-turn-helix motif that latches onto DNA through three
of the five conserved tryptophans. The motif selects a
short GAAA core sequence through an obliquely angled
recognition helix, with an accompanying bending of the DNA
axis in the direction of the protein. Together, these
features suggest a basis for the occurrence of GAAA repeats
within IRF response elements and provide clues to the
assembly of the higher-order interferon-beta
enhancesome.
