Summary information and primary citation
- PDB-id
-
1iaw;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- hydrolase-DNA
- Method
- X-ray (2.4 Å)
- Summary
- Crystal structure of naei complexed with 17mer DNA
- Reference
-
Huai Q, Colandene JD, Topal MD, Ke H (2001): "Structure
of NaeI-DNA complex reveals dual-mode DNA recognition and
complete dimer rearrangement."
Nat.Struct.Biol., 8, 665-669.
doi: 10.1038/90366.
- Abstract
- NaeI, a novel DNA endonuclease, shows topoisomerase and
recombinase activities when a Lys residue is substituted
for Leu 43. The NaeI-DNA structure demonstrates that each
of the two domains of NaeI recognizes one molecule of DNA
duplex. DNA recognition induces dramatic rearrangements:
narrowing the binding site of the Topo domain 16 A to grip
DNA, widening that of the Endo domain 8 A to encircle and
bend DNA 45 degrees for cleavage, and completely rebuilding
the homodimer interface. The NaeI-DNA structure presents
the first example of novel recognition of two copies of one
DNA sequence by two different amino acid sequences and two
different structural motifs in one polypeptide.
