Summary information and primary citation
- PDB-id
-
1hjc;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (2.65 Å)
- Summary
- Crystal structure of runx-1-aml1-cbfalpha runt domain
bound to a DNA fragment from the csf-1r promoter
- Reference
-
Tahirov TH, Inoue-Bungo T, Morii H, Fujikawa A, Sasaki M,
Kimura K, Shiina M, Sato K, Kumasaka T, Yamamoto M, Ishii
S, Ogata K (2001): "Structural
Analyses of DNA Recognition by the Aml1/Runx-1 Runt
Domain and its Allosteric Control by Cbfbeta."
Cell(Cambridge,Mass.), 104,
755. doi: 10.1016/S0092-8674(01)00271-9.
- Abstract
- The core binding factor (CBF) heterodimeric
transcription factors comprised of AML/CBFA/PEBP2alpha/Runx
and CBFbeta/PEBP2beta subunits are essential for
differentiation of hematopoietic and bone cells, and their
mutation is intimately related to the development of acute
leukemias and cleidocranial dysplasia. Here, we present the
crystal structures of the AML1/Runx-1/CBFalpha(Runt
domain)-CBFbeta(core domain)-C/EBPbeta(bZip)-DNA,
AML1/Runx-1/CBFalpha(Runt domain)-C/EBPbeta(bZip)-DNA, and
AML1/Runx-1/CBFalpha(Runt domain)-DNA complexes. The
hydrogen bonding network formed among CBFalpha(Runt domain)
and CBFbeta, and CBFalpha(Runt domain) and DNA revealed the
allosteric regulation mechanism of CBFalpha(Runt
domain)-DNA binding by CBFbeta. The point mutations of
CBFalpha related to the aforementioned diseases were also
mapped and their effect on DNA binding is discussed.
