SNAP annotations: PDB entry 1gt0

PDB-id

1gt0; DSSR-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

X-ray (2.6 Å)

Summary

Crystal structure of a pou-hmg-DNA ternary complex

Reference

Remenyi A, Lins K, Nissen LJ, Reinbold R, Scholer HR, Wilmanns M (2003): "Crystal Structure of a POU/Hmg/DNA Ternary Complex Suggests Differential Assembly of Oct4 and Sox2 on Two Enhancers." Genes Dev., 17, 2048. doi: 10.1101/GAD.269303.

Abstract

Members of the POU and SOX transcription factor families exemplify the partnerships established between various transcriptional regulators during early embryonic development. Although functional cooperativity between key regulator proteins is pivotal for milestone decisions in mammalian development, little is known about the underlying molecular mechanisms. In this study, we focus on two transcription factors, Oct4 and Sox2, as their combination on DNA is considered to direct the establishment of the first three lineages in the mammalian embryo. Using experimental high-resolution structure determination, followed by model building and experimental validation, we found that Oct4 and Sox2 were able to dimerize onto DNA in distinct conformational arrangements. We demonstrate that the DNA enhancer region of their target genes is responsible for the correct spatial alignment of glue-like interaction domains on their surface. Interestingly, these surfaces frequently have redundant functions and are instrumental in recruiting various interacting protein partners.