Summary information and primary citation
- PDB-id
-
1gcc;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- transcription-DNA
- Method
- NMR
- Summary
- Solution NMR structure of the complex of gcc-box
binding domain of aterf1 and gcc-box DNA, minimized average
structure
- Reference
-
Allen MD, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M
(1998): "A novel
mode of DNA recognition by a beta-sheet revealed by the
solution structure of the GCC-box binding domain in
complex with DNA." EMBO J.,
17, 5484-5496. doi: 10.1093/emboj/17.18.5484.
- Abstract
- The 3D solution structure of the GCC-box binding domain
of a protein from Arabidopsis thaliana in complex with its
target DNA fragment has been determined by heteronuclear
multidimensional NMR in combination with simulated
annealing and restrained molecular dynamic calculation. The
domain consists of a three-stranded anti-parallel
beta-sheet and an alpha-helix packed approximately parallel
to the beta-sheet. Arginine and tryptophan residues in the
beta-sheet are identified to contact eight of the nine
consecutive base pairs in the major groove, and at the same
time bind to the sugar phosphate backbones. The target DNA
bends slightly at the central CG step, thereby allowing the
DNA to follow the curvature of the beta-sheet.
