SNAP annotations: PDB entry 1fos

PDB-id

1fos; DSSR-derived features in text and JSON formats; DNAproDB

Class

transcription-DNA

Method

X-ray (3.05 Å)

Summary

Two human c-fos:c-jun:DNA complexes

Reference

Glover JN, Harrison SC (1995): "Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA." Nature, 373, 257-261. doi: 10.1038/373257a0.

Abstract

The Fos and Jun families of eukaryotic transcription factors heterodimerize to form complexes capable of binding 5'-TGAGTCA-3' DNA elements. We have determined the X-ray crystal structure of a heterodimer of the bZIP regions of c-Fos and c-Jun bound to DNA. Both subunits form continuous alpha-helices. The carboxy-terminal regions form an asymmetric coiled-coil, and the amino-terminal regions make base-specific contacts with DNA in the major groove. Comparison of the two crystallographically distinct protein-DNA complexes show that the coiled-coil is flexibly joined to the basic regions and that the Fos-Jun heterodimer does not recognize the asymmetric 5'-TGAGTCA-3' recognition element in a unique orientation. There is an extensive network of electrostatic interactions between subunits within the coiled-coil, consistent with proposals that these interactions determine preferential formation of the heterodimer over either of the homodimers.