Summary information and primary citation
- PDB-id
-
1etg;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- viral protein-RNA
- Method
- NMR
- Summary
- Rev response element (rre) RNA complexed with rev
peptide, NMR, 19 structures
- Reference
-
Battiste JL, Mao H, Rao NS, Tan R, Muhandiram DR, Kay LE,
Frankel AD, Williamson JR (1996): "Alpha
helix-RNA major groove recognition in an HIV-1 rev
peptide-RRE RNA complex." Science,
273, 1547-1551.
- Abstract
- The solution structure of a human immunodeficiency
virus type-1 (HIV-1) Rev peptide bound to stem-loop IIB of
the Rev response element (RRE) RNA was solved by nuclear
magnetic resonance spectroscopy. The Rev peptide has an
alpha-helical conformation and binds in the major groove of
the RNA near a purine-rich internal loop. Several arginine
side chains make base-specific contacts, and an asparagine
residue contacts a G.A base pair. The phosphate backbone
adjacent to a G.G base pair adopts an unusual structure
that allows the peptide to access a widened major groove.
The structure formed by the two purine-purine base pairs of
the RRE creates a distinctive binding pocket that the
peptide can use for specific recognition.
