Summary information and primary citation
- PDB-id
-
1e7k;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- RNA binding protein
- Method
- X-ray (2.9 Å)
- Summary
- Crystal structure of the spliceosomal 15.5kd protein
bound to a u4 snrna fragment
- Reference
-
Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R
(2000): "Crystal
Structure of the Spliceosomal 15.5Kd Protein Bound to a
U4 Snrna Fragment." Mol.Cell,
6, 1331. doi: 10.1016/S1097-2765(00)00131-3.
- Abstract
- We have determined the crystal structure of a
spliceosomal RNP complex comprising the 15.5kD protein of
the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4
snRNA. The protein interacts almost exclusively with a
purine-rich (5+2) internal loop within the 5' stem-loop,
giving an unusual RNA fold characterized by two tandem
sheared G-A base pairs, a high degree of purine stacking,
and the accommodation of a single RNA base, rotated out of
the RNA chain, in a pocket of the protein. Apart from
yielding the structure of an important entity in the
pre-mRNA splicing apparatus, this work also implies a model
for the complex of the 15.5kD protein with box C/D snoRNAs.
It additionally suggests a general recognition principle in
a novel family of RNA binding proteins.
