Summary information and primary citation
- PDB-id
-
1cma;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (2.8 Å)
- Summary
- Met repressor-DNA complex + s-adenosyl-methionine
- Reference
-
Somers WS, Phillips SE (1992): "Crystal
structure of the met repressor-operator complex at 2.8 A
resolution reveals DNA recognition by beta-strands."
Nature, 359, 387-393. doi:
10.1038/359387a0.
- Abstract
- The crystal structure of the met repressor-operator
complex shows two dimeric repressor molecules bound to
adjacent sites 8 base pairs apart on an 18-base-pair DNA
fragment. Sequence specificity is achieved by insertion of
double-stranded antiparallel protein beta-ribbons into the
major groove of B-form DNA, with direct hydrogen-bonding
between amino-acid side chains and the base pairs. The
repressor also recognizes sequence-dependent distortion or
flexibility of the operator phosphate backbone, conferring
specificity even for inaccessible base pairs.
