Summary information and primary citation
- PDB-id
-
1cgp;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (3.0 Å)
- Summary
- Catabolite gene activator protein (cap)-DNA complex +
adenosine-3',5'-cyclic-monophosphate
- Reference
-
Schultz SC, Shields GC, Steitz TA (1991): "Crystal
structure of a CAP-DNA complex: the DNA is bent by 90
degrees." Science, 253,
1001-1007.
- Abstract
- The 3 angstrom resolution crystal structure of the
Escherichia coli catabolite gene activator protein (CAP)
complexed with a 30-base pair DNA sequence shows that the
DNA is bent by 90 degrees. This bend results almost
entirely from two 40 degrees kinks that occur between TG/CA
base pairs at positions 5 and 6 on each side of the dyad
axis of the complex. DNA sequence discrimination by CAP
derives both from sequence-dependent distortion of the DNA
helix and from direct hydrogen-bonding interactions between
three protein side chains and the exposed edges of three
base pairs in the major groove of the DNA. The structure of
this transcription factor--DNA complex provides insights
into possible mechanisms of transcription activation.
