Summary information and primary citation
- PDB-id
-
1bp7;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (3.0 Å)
- Summary
- Group i mobile intron endonuclease i-crei complexed
with homing site DNA
- Reference
-
Jurica MS, Monnat Jr RJ, Stoddard BL (1998): "DNA
recognition and cleavage by the LAGLIDADG homing
endonuclease I-CreI." Mol.Cell,
2, 469-476. doi: 10.1016/S1097-2765(00)80146-X.
- Abstract
- The structure of the LAGLIDADG intron-encoded homing
endonuclease I-CreI bound to homing site DNA has been
determined. The interface is formed by an extended, concave
beta sheet from each enzyme monomer that contacts each DNA
half-site, resulting in direct side-chain contacts to 18 of
the 24 base pairs across the full-length homing site. The
structure indicates that I-CreI is optimized to its role in
genetic transposition by exhibiting long site-recognition
while being able to cleave many closely related target
sequences. DNA cleavage is mediated by a compact pair of
active sites in the I-CreI homodimer, each of which
contains a separate bound divalent cation.
