Summary information and primary citation
- PDB-id
-
1bgb;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- hydrolase-DNA
- Method
- X-ray (2.0 Å)
- Summary
- Ecorv endonuclease complex with 5'-cgggatatccc DNA
- Reference
-
Horton NC, Perona JJ (1998): "Recognition
of flanking DNA sequences by EcoRV endonuclease involves
alternative patterns of water-mediated contacts."
J.Biol.Chem., 273, 21721-21729.
doi: 10.1074/jbc.273.34.21721.
- Abstract
- The 2.1-A cocrystal structure of EcoRV endonuclease
bound to 5'-CGGGATATCCC, in a crystal lattice isomorphous
with the cocrystallized undecamer 5'-AAAGATATCTT previously
determined, shows novel base recognition in the major
groove of the DNA flanking the GATATC target site. Lys104
of the enzyme interacts through water molecules with the
exocyclic N-4 amino groups of flanking cytosines. Steric
exclusion of water molecule-binding sites by the 5-methyl
group of thymine drives the adoption of alternative
water-mediated contacts with AT versus GC flanks. This
structure provides a rare example of structural
adaptability in the recognition of different DNA sequences
by a protein and suggests preferred strategies for the
expansion of target site specificity by EcoRV.
