Summary information and primary citation
- PDB-id
-
1bf4;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- DNA-binding protein-DNA
- Method
- X-ray (1.6 Å)
- Summary
- Chromosomal DNA-binding protein sso7d-d(gcgaacgc)
complex
- Reference
-
Gao YG, Su SY, Robinson H, Padmanabhan S, Lim L, McCrary
BS, Edmondson SP, Shriver JW, Wang AH (1998): "The crystal
structure of the hyperthermophile chromosomal protein
Sso7d bound to DNA." Nat.Struct.Biol.,
5, 782-786. doi: 10.1038/1822.
- Abstract
- Sso7d and Sac7d are two small (approximately 7,000 Mr),
but abundant, chromosomal proteins from the
hyperthermophilic archaeabacteria Sulfolobus solfataricus
and S. acidocaldarius respectively. These proteins have
high thermal, acid and chemical stability. They bind DNA
without marked sequence preference and increase the Tm of
DNA by approximately 40 degrees C. Sso7d in complex with
GTAATTAC and GCGT(iU)CGC + GCGAACGC was crystallized in
different crystal lattices and the crystal structures were
solved at high resolution. Sso7d binds in the minor groove
of DNA and causes a single-step sharp kink in DNA
(approximately 60 degrees) by the intercalation of the
hydrophobic side chains of Val 26 and Met 29. The
intercalation sites are different in the two complexes.
Observations of this novel DNA binding mode in three
independent crystal lattices indicate that it is not a
function of crystal packing.
