Summary information and primary citation
- PDB-id
-
1bc7;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (2.01 Å)
- Summary
- Serum response factor accessory protein 1a (sap-1)-DNA
complex
- Reference
-
Mo Y, Vaessen B, Johnston K, Marmorstein R (1998):
"Structures
of SAP-1 bound to DNA targets from the E74 and c-fos
promoters: insights into DNA sequence discrimination by
Ets proteins." Mol.Cell, 2,
201-212. doi: 10.1016/S1097-2765(00)80130-6.
- Abstract
- SAP-1 is a member of the Ets transcription factors and
cooperates with SRF protein to activate transcription of
the c-fos protooncogene. The crystal structures of the
conserved ETS domain of SAP-1 bound to DNA sequences from
the E74 and c-fos promoters reveal that a set of conserved
residues contact a GGA core DNA sequence. Discrimination
for sequences outside this core is mediated by DNA contacts
from conserved and nonconserved protein residues and
sequence-dependent DNA structural properties characteristic
of A-form DNA structure. Comparison with the related
PU.1/DNA and GABPalpha/beta/DNA complexes provides general
insights into DNA discrimination between Ets proteins.
Modeling studies of a SAP-1/SRF/DNA complex suggest that
SRF may modulate SAP-1 binding to DNA by interacting with
its ETS domain.
