Summary information and primary citation
- PDB-id
-
10zy;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- ribosome
- Method
- cryo-EM (2.74 Å)
- Summary
- Yeast co-transcriptional pre-60s assembly intermediate
pwp1 rnp
- Reference
-
Piwowarczyk R, Klinge S (2026): "Mechanism
for the initiation of co-transcriptional pre-60 S
assembly." Biorxiv. doi: 10.64898/2026.05.22.727207.
- Abstract
- Eukaryotic ribosomal large subunit (60
<i>S</i> ) assembly requires an internal
transcribed spacer 2 (ITS2) to license both nucleolar and
nuclear pre-60 <i>S</i> assembly intermediates.
The underlying molecular mechanisms responsible for
nucleation of pre-60 <i>S</i> assembly, quality
control, and installation of ITS2 during co-transcriptional
stages remain unknown. Here we report the earliest
co-transcriptional assembly intermediates of the eukaryotic
60 <i>S</i> subunits. Together with biochemical
assays, our data reveal the architecture of
co-transcriptional pre-60 <i>S</i> assembly
initiation and progression, as well as the molecular logic
of an assembly checkpoint. This study highlights an
evolutionary solution by which complex RNA folding
processes can be parallelized and integrated via biological
AND-gating.
